Figure 1.

Map and expression of the MT-DNIIR transgene. (A) The human TGF-β type II receptor (TβR-II) was cytoplasmically truncated to form the dominant-negative TGF-β type II receptor (DNIIR), which was placed under the control of four metal-responsive elements (4× MRE) to generate the MT-DNIIR transgene. Arrows indicate the PCR primer sites. (B) RT-PCR analysis of total RNA from the mammary glands of wild-type and MT-DNIIR-28 mice treated with or without 25 mM zinc sulfate in the drinking water. GAPDH was amplified to control for loading errors.