Figure 2.

Increased specific activity of Tg-HDL in the presence of human apoA-I and Hpr. (A) Western blot with serial dilutions of plasma from HuapoA-I mice expressing Hpr and apoL-I from a single plasmid (HuapoA-I:Hpr:apoL-I) and normal human plasma. Hpr and apoL-I were detected with monoclonal antibodies. (B) Survival kinetics of naive mice infected with T. b. brucei ILTat 1.25 and then given 300 μl plasma i.v. from HuapoA-I mice expressing Hpr (black diamond; n = 3), apoL-I (black square; n = 3), or both Hpr and apoL-I from a single plasmid (black circle, Hpr:apoL-I; n = 3). The protection obtained by normal human plasma (dilution 1/8) is indicated by the inverted triangle. (C) A₂₈₀ profile of KBr-purified lipoproteins from human (dashed line) and HuapoA-I mouse plasma expressing Hpr:apoL-I (red line), Hpr (green line), and apoL-I (blue line) separated by size on a Superdex 200 column; fractions 6–7 are void, fractions 8–9 are human LDL, fractions 10–14 are HDLs, and fraction 15 is albumin. (D) Western blot of different size-fractionated lipoproteins from HuapoA-I mice expressing Hpr only (HuapoA-I:Hpr), apoL-I only (HuapoA-I:apoL-I), or Hpr and apoL-I from the same plasmid (HuapoA-I:Hpr:apoL-I). Hpr and apoL-I were detected with monoclonal antibodies. (E) In vitro lytic activity of size-fractionated HDL (fraction 11) from human and HuapoA-I mice expressing Hpr, apoL-I, or both from a single plasmid (dark gray bars). Open bars represent the lytic activity obtained in the presence of NH₄Cl (10 mM). Data show the mean and SEM of five independent measurements.