Table 2.
Melting temperatures of solution-phase unmodified DNA in the presence of triplex DNA binders: coralyne (CORA) and benzo[e]pyridoindole (BePI) and duplex DNA binders: 4′,6-diamidino-2-phenylindole (DAPI), ellipticine (EIPT), Amsacrine (AMSA), daunorubicin (DNR), anthraquinone-2-carboxylic acid (AQ2A), ethidium bromide (EB), and 9-aminoacridine (9-AA). Tm3-2 indicates a melting transition from a triplex to a duplex. Tm2-1 indicates a melting transition from a duplex to single stranded DNA.
| Compounds | Tm3-2 (°C) | Tm 2-1 (°C) |
|---|---|---|
| Control | ND | 58.9 |
| 9-AA | ND | 59.1 |
| AQ2A | ND | 59.1 |
| AMSA | ND | 59.2 |
| EIPT | ND | 59.3 |
| EB | ND | 59.8 |
| CORA | 17.1 | 60.1 |
| BePI | 34.8 | 61.4 |
| DNR | ND | 61.7 |
| DAPI | ND | 66.3 |