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. Author manuscript; available in PMC: 2008 Aug 26.
Published in final edited form as: Inorg Chem. 2007 Mar 28;46(9):3420–3422. doi: 10.1021/ic0619714

Figure 1.

Figure 1

Cleavage of supercoiled pUC18 DNA (0.154 mM) by P4+ in 7 mM Na3PO4 buffer (pH 7.0) at 25°C. Lane M, marker lane containing Form I, II and III DNA; lane 1, DNA control; lane 2, DNA + P4+ (0.0128 mM); lane 3, DNA + GSH (0.513 mM); lane 4, DNA + GSH (0.256 mM) + P4+ (0.0128 mM) under aerobic conditions; lane 5, same as lane 4 under anaerobic conditions; lane 6, DNA + GSH (0.512 mM) + Fe-Blm (0.0128 mM) under aerobic conditions; lane 7, same as lane 6 under anaerobic conditions. Incubation time for all these cases was limited for 1 hour.