Fig. 5.

Effects of TNF-α on NF-κB transcription in L6 myotubes (A) and myoblasts (B). L6 myoblasts were transfected with NF-κB-dependent reporter plasmid and Renilla control plasmid. After transfection, cells were exposed to increasing concentrations of TNF-α for 12 h. Differentiation of myoblasts into myotubes was performed following transfection. To correct for variation in transfection efficiency, values of the luciferase assay were normalized with measured renilla activities. Luciferase assays in individual transfections were performed in duplicate. Results shown are the mean of three independent experiments ± S.E.M.