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. Author manuscript; available in PMC: 2008 Aug 27.
Published in final edited form as: Steroids. 2007 Jun 2;72(9-10):705–712. doi: 10.1016/j.steroids.2007.05.007

Fig. 6.

Fig. 6

Effects of TNF-α and DEX on NF-κB transcription in L6 myotubes (A) and myoblasts (B). L6 myoblasts were transfected with a NF-κB-dependent reporter plasmid and Renilla control plasmid. After transfection, cells were exposed to 20 ng/ml of TNF-α for 12h followed by DEX 10−6M for 6h. Differentiation of myoblasts into myotubes was performed following transfection. To correct for variation in transfection efficiency, values of the luciferase assay were normalized with measured renilla activities. Luciferase assays in individual transfections were performed in duplicate. Results shown are the mean of three independent experiments ± S.E.M.