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. 2008 Sep 2;118(10):3343–3354. doi: 10.1172/JCI34871

Figure 7. APJ receptor regulates Ang II signaling via AT1R independent of apelin.

Figure 7

(A) Western blotting of cells transfected with AT1R alone or with APJ revealed decreased phosphorylation of ERK1/2 in cells coexpressing APJ. The bottom panel shows control blot with total ERK1 antibody. (B) NF-κB and SRE reporter constructs transfected with an AT1R expression construct and varying amounts of APJ expression construct showed decreased signaling with greater APJ/AT1R ratios. Graphs represent mean ± SEM. (C) Coexpression of APJ did not inhibit vasopressin-induced nuclear signaling by the vasopressin receptors (AVPR1A and AVPR2) through the NF-κB consensus reporter. (D) Luciferase analysis using an NF-κB reporter construct with siRNA directed against APJ demonstrated enhanced Ang II response compared with control siRNA (*P < 0.05, †P < 0.01).