Figure 5.

The stimulation of [³H]GDP dissociation from rab5 by CCVs is not affected by wortmannin. REP-1/[³H]GDP–rab5 (100 nM) was incubated in the absence (•) or presence (▵,▾) of 1 mg/ml CCVs for the indicated periods of time in the absence (▾) or presence (▵) of 200 nM wortmannin. At the end of the reaction the samples were loaded on filters and protein-bound radioactivity was quantified as described in MATERIALS AND METHODS. The data represent the mean values from two independent experiments of filter- associated counts expressed relative to counts measured before incubation.