FIG. 1.
Response to local acetylcholine (ACh) application. A: schematic diagram of experimental setup, with extracellular ventral root and spinal cord electrodes recording overall activity and an intracellular microelectrode recording individual cells. Example of responses in Ringers (Bi) and after perfusion of nicotinic antagonist mecamylamine (20 μM; Bii) had attenuated the excitatory responses, revealing a hyperpolarizing response. In another experiment the responses observed in Ringers (Ci) and after perfusion of muscarinic antagonist scopolamine (10 μM; Cii) showing a slightly stronger response.