FIG. 2.
Neurons are depolarized directly by ACh application. Ai: a lateral interneuron depolarized and received increased synaptic input after ACh was applied in Ringers. Aii: depolarization persisted in the lateral interneuron after perfusion of 3 μM tetrodotoxin (TTX). Bi: depolarization of a motoneuron in TTX. Bii: depolarization persisted after perfusion of muscarinic antagonist scopolamine (10 μM). Ci: depolarization of an unidentified neuron in TTX. Cii: depolarization was blocked after perfusion of nicotinic antagonist mecamylamine (20 μM).