Figure 11.

Effect of hyperosmolarity on ENaC currents in cytochalasin B-pretreated oocytes. ENaC expressing oocytes pretreated with cytochalasin B for 2–5 h (A) were not responsive to a 45-min incubation in 260 mosmol hyperosmotic solution (B). Indeed, the shrinking-inhibitable currents (C ) were much smaller than those observed in the absence of cytochalasin pretreatment (Fig. 9 C ) and may be explained by the small baseline drift observed during the control period (see Fig. 12). The amiloride-insensitive currents recorded at the end of the experiment were not affected by cytochalasin pretreatment (D).