Figure 15.

Summary data indicating the lack of effect of a 5 in. H₂O pipette pressure difference on ENaC's open probability. As observed from the individual experiment data (A), there were no reproducible effects of negative or positive pipette pressure on P _o. Examination of the P _o in individual experiments as illustrated in Fig. 14 indicated that none of these experiments exhibited any measurable changes of activity immediately after applying either the positive or negative pipette pressures. The data from these nine experiments are summarized in B and also indicate the lack of significant effect of pipette pressure difference on the channel's mean open probability (P > 0.3 between all four groups).