Figure 6.

ENaC currents are also insensitive to osmotic cell swelling in the presence of cytochalasin B. Oocytes were pretreated with cytochalasin B for 2–5 h. Currents are summarized at a holding voltage of −100 mV. In this group of experiments there were no differences between the 0 min control currents recorded in the 210-mosmol solution and +30 min currents recorded 30 min after incubation in the 160-mosmol solution. There were also no differences between the amiloride-insensitive currents in oocytes treated with cytochalasin B and those untreated (see 10 μM amiloride in Fig. 4). n = 6.