Figure 6.

Confocal microscopic images demonstrating that cleavage at D⁴²¹, phosphorylation and the conformational Alz-50 epitope colocalize in Alzheimer’s disease (AD) brain. Triple labelling immunofluorescence with Tau C3, Alz-50 and pSer³⁹⁶. Neurofibrillary tangles (NFTs) with truncated tau at D⁴²¹ and phosphorylation at Ser³⁹⁶ were found (white–blue NFTs, d); as well as NFT with phosphorylation at site Ser³⁹⁶, cleavaged at site D⁴²¹ and the Alz-50 conformational change (white NFTs, d); and finally NFTs with nothing but phosphorylation at site Ser³⁹⁶ (blue NFTs, Figure 4d).