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Representative gel of cyclophilin to determination the number cycles during PCR. Determination of number of cycles for the cyclophilin (1 = pattern molecular weight, 2 = 16 cycles; 3 = 20 cycles; 4 = 24 cycles; 5 = 28 cycles, 6 = 32 cycles; 7 = 36 cycles; 8 = 40 cycles). Product of cyclophilin PCR with 440 bp. We used 32 cycles because of the exponential phase.
