Figure 5.

Movie 5: GFP-vimentin fragments—possible intermediates in IF turnover. (A) The coalignment of two GFP-vimentin fragments with an IF is observed. Every other frame from the first 10 min of the movie is shown, with the IFs of interest outlined. In the first frame the fragments of interest are outlined as they are in the movie. Bar, 5 μm. (B) We observed the formation of two fragments from a longer fragment. The six frames of the movie are shown. As in A, the fragment(s) of interest are outlined. Bar, 5 μm.