Figure 5.

Time course of S100b-Cre-mediated deletion of Cx43^fl allele in the postnatal cerebellum. (A) Cre-mediated recombination was assayed by whole mount lacZ staining of parasagittal brain slices from Cx43^fl/+:S100b-Cre mice and positive control Cx43^del/+ mice at P14, P21, and P28. No lacZ-positive cells were detected in negative control Cx43^fl/+ mice at those stages (data not shown). Scale bar, 1 mm. (B) Immunofluorescence analysis of parasagittal sections of the cerebellum from 2-month-old Cx43^fl/+:S100b-Cre mice and positive control Cx43^del/+ mice using antibodies directed to β-galactosidase. β-galactosidase immunoreactivity was localized in the nucleus. Arrowheads indicate Purkinje cell layer. gcl, granule cell layer; ml, molecular layer; wm, white matter. Scale bars, 200 μm.