Figure 3.

(A) Timecourse of fPSP amplitude and example traces (i) at baseline, (ii) following application of CRF (0.1 μM), (iii) following washout of the CRF and application of CNQX (10 μM), and (iv) following application of TTX (100 nM). Each symbol represents the fPSP amplitude for a single response. Horizontal scale bar 10 min. (B) Example of evoked field potentials before (black trace) and after (grey trace) the application of CRF (0.1 μM); the non-biological component that was not sensitive to TTX applied at the end of the experiment has been digitally removed. Dotted lines indicate the method for determining the fPSP amplitude. (C) The mean fPSP amplitude evoked by currents at 60–80% of maximum at baseline and after 15 min incubation with CRF (0.1 μM). Amplitude was determined from responses similar to that shown in (B). **p < 0.01, paired t-test, n = 7 slices, 5 rats. (D) Stimulus current-response relationship for evoked fPSPs in the absence (•) and presence (∘) of CRF (0.1 μM), statistical analysis was carried using a two-way repeated measures ANOVA (see text) followed by a post-hoc Bonferroni test (*p < 0.05, **p < 0.01, n = 7 slices, 5 rats). (E) Paired-pulse relationship for evoked fPSPs in the absence (•) and presence (∘) or CRF (0.1 μM), statistical analysis was carried out using a two-way repeated-measures ANOVA (see text; n = 8 slices, 5 rats).