Figure 1.

Neuronal differentiation of hESCs through the SDIA method. (A) Human embryonic stem cells (hESCs) growing on human foreskin fibroblasts (hFFs). (B) Differentiated hESCs on PA6 cells. (C–F) Expression of the early neuronal marker Nestin by the hESC-derived cells, identified by the expression of HNuc, after differentiation for 3 weeks on PA6 cells. Detection of fibroblast growth factor receptor (FGFR)-1 in: (G–I) hESCs identified by the expression of the proliferation marker Ki67; (J–L) a monolayer of PA6 cells; and (M–O) hESC-derived TH-expressing neurons. Distance bars: A–E, G, H: 200 μm; F: 100 μm; I, M–O: 20 μm; J–L: 50 μm.