FIGURE 7.

shRNAs are more potent than artificial miRNAs in vivo. (A) Gene silencing efficacy in vivo was compared by coelectroporating SCA1 RNAi and RNAi luciferase reporter plasmids into tibialis anterior muscles of 6–8-wk-old mice. Low and high doses are 1:1 and 10:1 (RNAi:target) ratios, respectively. Renilla luciferase activity was measured in vivo using bioluminescence imaging after 8 d. Representative “heat-map” images are shown along with quantitative analysis (B) represented as mean±SEM (n =4; P < 0.05 within each dose). Similar silencing trends were also observed at 4 d post-treatment (data not shown).