FIGURE 7.

(A) A nucleotide sequence of the bat VES SINE copy used for preparation of constructs. The SINE and its flanking sequences are shown in upper and lower cases, respectively. TSD flanking SINE is underlined. In the VES sequence, a pol III promoter (box A and box B), a potential polyadenylation signal pA (underlined), and a pol III terminator (underlined with dotted line) are indicated. (B) The structure of two constructs used to study the polyadenylation ability of VES SINE pol III transcripts. The first 200 bp of VES are depicted as a rectangle, whereas a terminal region of the SINE constructs is represented as a nucleotide sequence. A potential polyadenylation signal is underlined; a terminator is underlined with a dotted line. Note that two additional T residues were introduced in the terminator of the both constructs. (C) Blot analysis of VES SINE transcripts isolated from HeLa cells transfected with VES-containing constructs with (VES-pA₁) or without (VES-pA₀) a polyadenylation signal. Long forms of VES RNA were indicated with a square bracket.