FIGURE 1.

Generation of Dazap1 mutant alleles. (A) Structure of the mouse Dazap1 gene and the scheme to generate the various mutant alleles. The restriction sites are (B) BamHI, (N) NotI, (R) EcoRI, (RV) EcoRV, (Sm) SmaI, and (X) XhoI. The loxP sites are depicted as close triangles. (B) Genotyping by Southern hybridization. Genomic DNAs from mice with the various genotypes were digested with BamHI and blotted with a probe from intron 7, indicated in A. The 20-, 9.5-, and 7.6-kb fragments are from the +, Fn, and F/null alleles, respectively. (C) PCR analyses of the Dazap1 alleles. Mouse tail DNA samples were assayed for the presence of loxP-a, loxP-b, and loxP-c using primers flanking the sites as indicated in A. (*) The heteroduplex in the PCR reaction of the loxP-a site.