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Immunoblotting of NE proteins of RV-SMC cells (A–C; 2.5 × 104 cell equivalents/lane) and pachytene spermatocytes of the rat (A′–C′; 2.5 × 104 cell equivalents/lane) that were fractionated with buffers containing 1% Triton X-100 and 250 mM or 2 M salt. mAbs 13d4 (A–A′; against LAPs2 α, β, and γ), PKB8 (B–B′; against lamins A, B1, and C) as well as R27 (C–C′; against lamins A, C, and C2) were used to detect the respective antigens. S, Supernatant fraction; P, pellet fraction.
