Figure 6.

Local knockout of NR1 gene mediated by injection of lentivector encoding GFP and Cre into gene-targeted fNR1 mice. (A) Insertion of loxP elements in the Grin1 locus of mouse chromosome 2 (above) enable Cre-mediated KO of the gene. LoxP elements were inserted into intron sequences of the gene (below) allowing normal gene function in the fNR1 mouse in the absence of Cre (modified from Tsien et al., 1996b). (B) After transduction by lentivector encoding GFP and Cre, NR1 mRNA is found locally depleted at the injection site by in situ hybridization on a 16 m cryostat section. Blue signal is from NBT/BCIP detection of the digoxigenin-labeled antisense probe, while the pink signal is from the ISH Red counterstain (Ventana Medical Systems, Inc.).