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. Author manuscript; available in PMC: 2009 Aug 8.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jun 2;372(4):746–751. doi: 10.1016/j.bbrc.2008.05.098

Fig. 2. GW4064 enhanced decorin mRNA expression at the transcriptional level.

Fig. 2

(A) Actinomycin D substantially inhibited GW4064-mediated upregulation of decorin mRNA. RASMCs were treated with actinomycin D (5µg/mL) for 30 min prior to GW4064 treatment. Twenty-four hours later, Dcn mRNA expression in RASMCs was similarly examined by real-time RT–PCR. In a separate experiment, a series of human Dcn promoter luciferase reporter constructs (shown in panel B) were transiently transfected in CV-1 cells. After 12h, the transfected cells were treated for 24h with different concentrations of GW4064 or vehicle DMSO. The luciferase activity was normalized against the β-gal activity, and the data are reported as fold induction observed with GW treatment relative to DMSO control. Shown in panels C, D, and E are data from transfection with a reporter driven by a Dcn promoter of 3275bp, 2620 bp, and 1261 bp, respectively.