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. Author manuscript; available in PMC: 2008 Aug 27.
Published in final edited form as: Biochemistry. 2007 Apr 4;46(17):5038–5049. doi: 10.1021/bi700147b

Table 4.

5′-3H Kinetic Isotope Effect and Binding Isotope Effect Data

Enzyme V/K KIE BIE Cf Intrinsic KIE
Native PNP 1.054 ± 0.002a 1.015 ± 0.003 0.147a 1.046 ± 0.004
His257Phe 0.992 ± 0.003 1.022 ± 0.004 0.301 ± 0.010 0.968 ± 0.005
His257Gly 0.925 ± 0.005 1.024 ± 0.006 0.600 ± 0.021 0.859 ± 0.007
His257Asp 1.046 ± 0.004 0.980 ± 0.005 0.042 ± 0.002 1.069 ± 0.007
a

Value reported in reference (5).