Table 1.
Biomass yield, cell dry weight and side product concentration for E. coli W3110 during the different cultivation types on glucose based mineral salt medium.
| Type of cultivation | Addition of trace elements | YX/S1 (g g-1) | CDW (g L-1) | Acetate (mM) | Lactate (mM) | Formate (mM) | |||||
| Batch2 | Fed-batch3 | Batch | Fed-batch | Batch | Fed-batch | Batch | Fed-batch | Batch | Fed-batch | ||
| Control | + | 0.49 (0.01)4 | n.d. | 19.3 (1.1) | n.d. | 43.8 (10.1) | n.d. | 4.5 (6.3) | n.d. | 0.5 (0.7) | n.d. |
| Control | - | 0.48 (0.01) | 0.55 | 18.9 (0.4) | 36.4 | 30.8 (8.4) | 29.7 | 3.8 (3.4) | 11.6 | 1.8 (2.0) | 5.0 |
| STR-PFR | + | 0.50 | 37.8 | 0.1 | 2.2 | 0.0 | |||||
| STR-PFR | - | 0.45 | 36.4 | 4.5 | 0.0 | 14.8 | |||||
| Oxygen downshift | + | 0.23 | 20.2 | 139.7 | 435.8 | 0.0 | |||||
| Oxygen downshift | - | 0.38 | 24.7 | 173.2 | 60.6 | 16.9 | |||||
1 Yield coefficients were calculated for a time period of 4 hours starting with the feeding start. Here YX/S includes also biomass formed from acetate which is consumed during this phase.
2 Batch samples were taken at the end of the exponential growth phase.
3 Fed-batch sample was taken 4 h after the feed start.
4 Numbers in brackets indicate standard deviations between different cultivations.