Table 1.
Composition of the culture medium.
| Product | Concentration (g/L) | Product | Concentration (g/L) |
| Glucose | 20 | Isoleucine | 0.025 |
| Sodium acetate | 1 | Leucine | 0.05875 |
| Ammonium citrate | 0.6 | Valine | 0.04125 |
| KH2PO4 | 3 (9) | Cysteine | 0.17 |
| K2HPO4 | 2,5 (7,5) | Phenylalanine | 0.28 |
| MgCl2, 6H2O | 0.2 | Tyrosine | 0.29 |
| FeSO4, 7H2O | 0.011 | Adenine | 0.05 |
| CaCl2, 2H2O | 0.05 | Guanine | 0.05 |
| ZnSO4, 7H2O | 0.005 | Uracil | 0.05 |
| COCl2, 6H2O | 0.0025 | Xanthin | 0.05 |
| Alanine | 0.24 | P-aminobenzoic acid | 0.01 |
| Arginine | 0.12 | Biotin | 0.01 |
| Asparagine | 0.34 | Cyano-cobalamine (B12) | 0.001 |
| Glutamine | 0.51 | Folic acid | 0.01 |
| Glycine | 0.17 | Inosine | 0.025 |
| Histidine | 0.11 | Nicotinic acid | 0.001 |
| Lysine | 0.35 | Orotic acid | 0.005 |
| Methionine | 0.12 | Ca panthotenate | 0.001 |
| Proline | 0.68 | Pyridoxamine | 0.005 |
| Serine | 0.34 | Pyridoxin (B6) | 0.002 |
| Threonine | 0.23 | Riboflavin (B2) | 0.001 |
| Tryptophan | 0.05 | Thiamine | 0.001 |
| D, L-6,8-thioctic acid | 0.0025 | ||
| Thymidine | 0.025 | ||
The lines separating the different products indicate those that are dissolved together. Values into brackets in front of KH2PO4 and K2HPO4 indicate the required concentrations to obtain a buffered media for pre-cultures