Figure 4.

The effect of UV-B treatment on the expression of Nalp subtypes in HCE-T cells. Changes of Nalp mRNA expression 6 h (A) or 24 h (B) after UV-B treatment are shown. The relative expressions of Nalp1, Nalp2, Nalp3, Nalp7, and Nalp10 were measured in 85%–90% confluent HCE-T cells irradiated with 30 mJ/cm² UV-B as described in the legend of Figure 1 and are shown compared to cells incubated in the same way but not irradiated. Mean values and ±SD were calculated from six independent measurements. The asterisk indicates a p<0.005. Changes of Nalp1 protein expression 6 h (C) and 24 h (D) after UV-B irradiation of HCE-T cells are illustrated. Equal amount of sample loading was verified by detecting β-actin protein expression.