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. 1985 Nov;56(2):579–588. doi: 10.1128/jvi.56.2.579-588.1985

Isolation and molecular cloning of a fast-growing strain of human hepatitis A virus from its double-stranded replicative form.

A Venuti, C Di Russo, N del Grosso, A M Patti, F Ruggeri, P R De Stasio, M G Martiniello, P Pagnotti, A M Degener, M Midulla, et al.
PMCID: PMC252615  PMID: 2997478

Abstract

A fast-growing strain of human hepatitis A virus was selected and characterized. The virus has the unusual property of developing a strong cytopathic effect in tissue culture in 7 to 10 days. Sequences of the viral genome were cloned into recombinant plasmids with the double-stranded replicative form as a template for the reverse transcription of cDNA. Restriction analysis and direct sequencing indicate that this strain is different from that described by Ticehurst et al. (Proc. Natl. Acad. Sci. USA 80:5885-5889, 1983) in the region that presumptively codes for the major capsid protein VP1, but both isolates have conserved large areas of homology in the untranslated 5'-terminal sequences of the genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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