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. Author manuscript; available in PMC: 2008 Aug 28.
Published in final edited form as: J Phys Chem B. 2007 Jun 5;111(25):7415–7421. doi: 10.1021/jp071107c

Table 1. Sugar radicals formed via photo-excitation of one-electron oxidized dinucleoside phosphates and oligonucleotides at 143K a,b,c.

Compound Percent convertedd Init. Rate (%/min) C1′•e C3′• e C5′• e
No filter ≥540 nm
dGuof 90 4.2 3.5 10 35 55
8-D-dGuof 90 4.0 - 10 35 55
8-D-3′-dGMPf 85 1.9 - 40 - 60
8-D-3′-dGMP(77 K)f 15 0.08 - 40 - 60
5′-dGMPf 95 4.0 - 95 5 -
5′-dGMP (77 K)f 30 1.0 - 15 30 55
TpdGg 85 4.0 - 90 10 -
dGpdG 90 2.5 - 35 5 60
AGA 50 0.5 - 20 20 60
TGT 95 2.0 0.4 40 5 50
TGGT 75 1.2 0.7 30 10 60
5′-p-TGGT 80 1.2 0.8 30 10 60
TGGGT 70 1.0 - 60 - 40
AGGGAh 80 1.2 - 10h 20h 30h
TTGTT 75 0.8 - 50 10 40
TTGGTT 55 1.1 - 50 7 43
TTGGTTGGTT 55 ca. 0.6 0.15 60 - 40
dsDNA (ice) i 40 0.26 0.07 100 - -
a

Radical percentages expressed to ±10% relative error. Initial rates are based on the % G•+ converted to sugar radicals after the first 20 min of visible light exposure and should be consider as indicative of the relative rates of sugar radical formation.

b

All glassy samples are at the native pH of 7.5 M LiCl (ca. 5).14

c

All samples were illuminated at 143 K unless indicated otherwise.

d

Percentage of conversion of one-electron oxidized dinucleoside phosphate or oligonucleotide to sugar radicals. The total spectral intensities before and after illumination were the same, within experimental uncertainties.

e

Each calculated as percentage of total sugar radical concentration; these sum to 100%.

h

The overall spectrum obtained after photo-excitation has also an underlying unidentified spectrum (ca. 40%).

i

DNA (salmon testes) in a frozen aqueous (D2O) solution (ice).