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. 2008 Aug 20;3(8):e3010. doi: 10.1371/journal.pone.0003010

Figure 1. Ubiquitous expression of BbCRASP-2 in infected mice.

Figure 1

The relative expression levels of BbCRASP-2 in the murine host, and in representative life stages of ticks, are analyzed and presented as copies of BbCRASP-2 transcript per 1000 copies of flaB transcripts. Total RNA was isolated from multiple tissues of B. burgdorferi-infected mice (6 mice/group) at day 5, 7, 12, 16 and 24 following challenge, from infected fed larva (FL) after 3 days of feeding, unfed nymphs following larval molting (UN) and fed nymphs (FN) after 3 days of feeding. BbCRASP-2 transcripts were measured using quantitative RT-PCR. Error bars represent the mean ± SEM from four quantitative PCR analyses of two independent murine infection experiments. BbCRASP-2 transcripts were abundant in all murine tissues tested but were not detected in any stages of the ticks.