Skip to main content
. 2008 Aug 20;3(8):e3010. doi: 10.1371/journal.pone.0003010

Figure 5. BbCRASP-2 mutant B. burgdorferi retain full pathogenicity in the murine host.

Figure 5

The B. burgdorferi burdens in multiple tissues of the infected mice are shown. Mice (6 animals/group) were infected with either the wild type or the BbCRASP-2 mutant B. burgdorferi and spirochete burdens were analyzed as before by measuring copies of B. burgdorferi flaB RNA at day 7, 12 and 18 following infection. Amounts of murine ß-actin were determined in each sample and used to normalize the quantities of spirochete RNA. Bars represent the mean measurements ± SEM from four quantitative PCR measurements from two independent infection experiments. No difference between wild type and BbCRASP-2 mutant levels was statistically significant in any tissue or time point measured (P>0.05, n = 4).