Figure 2. Correlation of log₂ ratios resulting from comparative transcriptional analysis of the laboratory cultured A. fumigatus cell populations T0 and T60 under varying mRNA amplification protocols.

Correlation of technically duplicated log₂ ratios between competitive hybridisations using single (aRNA₁), double (aRNA₂) and unamplified (totRNA) RNA samples. (A and B) Correlation between log₂ ratios obtained using cDNA derived from amplified and total RNA (totRNA v aRNA_r1 r = 0.74–0.80, totRNA v aRNA_r2 r = 0.74–0.80) (C) Cross-protocol pairings revealed highest correlations between slides using cDNA derived from amplified RNA (aRNA_r1 v aRNA_r2 r = 0.88–0.91) Surprisingly, technical replicates of slides using cDNA derived from total RNA (totRNA r = 0.80) were comparable to cross-protocol pairings (data not shown).