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. 2008 Sep 1;205(9):1967–1973. doi: 10.1084/jem.20071632

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© 2008 Maelfait et al.

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Figure 5. — Recombinant caspase-8 cleaves pro–IL-1β in vitro. (A) [³⁵S]methionine-labeled pro–IL-1β was incubated for 1.5 h at 37°C with increasing concentrations of recombinant caspase-8 (10, 30, 100, and 300 ng) or caspase-1 (30 ng). Pro–IL-1β cleavage was revealed by SDS-PAGE followed by autoradiography. (B) [³⁵S]methionine-labeled pro–IL-1β WT or the indicated mutants were incubated for 1.5 h at 37°C with recombinant caspase-1, -3, or -8 (200 ng) and analyzed as in A.