Figure 5.

Evasin-1 inhibits cellular recruitment in models of Th1 and Th2 inflammation. (a) Evasin-1 inhibits granulocyte recruitment induced by antigen challenge of immunized mice in models of Th1-predominant delayed-type hypersensitivity and Th2-predominant late-phase response. In the Th1 model, 10 μg Evasin-1 was administered s.c. 45 min before intrapleural injection of 10 μg of mBSA in mice immunized with mBSA in Freund's complete adjuvant. Cells in the pleural cavity were counted 48 h after the injection of antigen. In the Th2 model, 10 μg Evasin-1 was administered s.c. 45 min before and 24 h after the intratracheal injection of 10 μg of S. mansoni egg antigen in mice immunized with S. mansoni eggs. Cells in the bronchoalveolar lavage fluid were counted 24 and 48 h after the injection of antigen. There were six mice per group in each experiment. *, P < 0.05; **, P < 0.01 when compared with PBS-treated group. (b) Evasin-1 blocks adhesion and emigration of leukocytes induced by mMIP-1α as assessed by intravital microscopy performed in the cremaster vein of C57BL/6 mice. Evasin-1 was injected s.c. in the right scrotal skin 1 h before exteriorization. Normalized data were analyzed by one-way analysis of variance, and differences between groups were assessed using Student-Newman-Keuls post-test. *, P < 0.05; **, P < 0.01. There were six mice per group in each experiment. Error bars represent the mean ± SEM.