Table I.
The MZ can replenish N− H-CDR3 from adult BM precursors
| Productive
|
Nonproductive
|
|||||
|---|---|---|---|---|---|---|
| Total H-CDR3 sequences | Number of N− H-CDR3 sequences |
Percentage N− | Total H-CDR3 sequences |
Number of N− H- CDR3 sequences |
Percentage N− | |
| MZ | 73 | 6 | 8 | 27 | 2 | 7 |
| FO | 43 | 0 | 0 | 17 | 0 | 0 |
Recipient mice (n = 2) were lethally irradiated, as described in Materials and methods, and reconstituted with B220-depleted BM precursors from littermate donors. After 5 wk of reconstitution, MZ and FO B cells were sorted as previously described. MZ and FO DNA was PCR amplified (four to eight independent PCRs), cloned, and sequenced, and the N− H-CDR3 were analyzed.