Figure 7.

Adoptive T cell transfer from WT and IL-6–deficient mice in SCID mice. (A) CD4⁺ T cells from WT and IL-6 donor mice were transferred into CB-17/SCID mice, followed by oxazolone sensitization and intrarectal oxazolone administration. The body weight of reconstituted mice was analyzed at the indicated time points. Mean values ± the SEM from one representative experiment are shown. Whereas mice reconstituted with WT T cells showed a marked weight loss, mice given IL-6–deficient T cells were protected from colitis. For this experiment, four SCID mice in each group were used. (B) Endoscopic assessment of colonic inflammation of SCID mice reconstituted with WT (left) or IL-6–deficient (right) T cells upon oxazolone administration. Endoscopy was performed 2 d after intrarectal oxazolone challenge. (C) Quantitative endoscopic analysis of inflammation (MEICS score) of SCID mice in oxazolone colitis was done 2 d after administration of oxazolone. A significantly (P < 0.05) lower endoscopic score was observed in SCID mice reconstituted with T cells from IL-6–deficient mice as compared with the WT group.