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. 2008 Sep 1;205(9):2139–2149. doi: 10.1084/jem.20080414

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© 2008 Jaensson et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — CD103⁺ DCs present orally administered soluble antigen to CD4⁺ and CD8⁺ T cells. (A and B) Mice received PBS 5 mg OVA i.p., or 50 mg orally, and MLN were harvested after 17 h. 10⁵ CD103⁺ and CD103⁻ MLN DCs were isolated by cell sorting and cocultured with 2 × 10⁵ CFSE-labeled OT-I or OT-II cells. (A) CFSE profile of OT-I and OT-II cells 3.5 d after coculture with indicated DCs. Data are representative of three to five separate experiments using MLN DCs isolated from 15–20 pooled mice per group. (B) Expression of CCR9 and α4β7 was analyzed by FACS after 3.5 d in coculture. The graph shows mean and SEM from five independent experiments. *, P < 0.05 (when comparing CD103⁺ and CD103⁻ DC–primed cultures).