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. 2008 Sep 1;205(9):2139–2149. doi: 10.1084/jem.20080414

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© 2008 Jaensson et al.

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Figure 6. — Phenotypic characterization of human CD103⁺ and CD103⁻ MLN DCs. (A) Human MLN section stained with anti–DC-LAMP (FITC), CD103 (Cy3), and IgG (Cy5) antibody to identify B cell follicles for orientation in the LN. CD103⁺ DCs (yellow). Bar, 100 μm. (B) Percent of DCs expressing CD103 in normal and Crohn's MLN, as determined by flow cytometry using the DC gatings described in Fig. S2 A (available at http://www.jem.org/cgi/content/full/jem.20080414/DC1). Filled squares, normal MLN (bladder reconstruction patients; n = 9); open squares, colon cancer patients (n = 4); triangles, SB Crohn's patients (n = 6). (C and D) CD40 and CD83 expression on CD103⁺ and CD103⁻ normal MLN DCs using the gatings described in Fig. S2 A (available at http://www.jem.org/cgi/content/full/jem.20080414/DC1). Representative FACS plots (C) and mean and SD (D) of three (CD83) or four (CD40) independent experiments. **, P < 0.01. Results are normalized within each experiment to the percent expression on CD103⁺ DCs.