Figure 3.

Multicolour fluorescence microscopy images showing caspase-3-positive cells detected 96 hr postinfection in BALB/c mice infected with Salmonella Typhimurium C5. (a) A caspase-3-positive cell containing no intracellular bacteria, (b) a caspase-3-positive cell harbouring an intracellular bacterium, (c) a caspase-3-positive cell containing many intracellular bacteria. (S. enterica were detected with Anti-Salmonella O4-LPS Antibody Congjugated to Alexa Fluor 488 and appear green, caspase-3-positive cells were detected with Anti-Caspase-3 antibody/Alexa Fluor 568 and appear red, nuclei are stained with DAPI and appear blue. Images were taken at magnification × 630).