Figure 4.

Upon restimulation dex MDC-primed T cells proliferate and produce IFN-γ comparable to control MDC-primed T cells. 15 × 10⁴ allogeneic T cells were primed with 20 × 10³ control or dex MDC. At day 7 the T cells were rested for 2 days in refreshed medium. T cells (75 × 10³) primed with control or dex MDC were restimulated with 75 × 10² mature mo-MDC originating from the same donor as the control- and dex MDC. (a) T-cell proliferation was assessed by [³H]-thymidine incorporation 4 days after restimulation of the control or dex MDC primed T cells. (b) IFN-γ production in the supernatant on day 4 of restimulation was determined by ELISA. Data represent mean with SEM of three separate experiments.