Figure 6.
TLR2-induced cytokine release in enriched monocytes is in part inhibited by ISO-1 pretreatment. Monocytes were enriched by negative magnetic selection from human peripheral blood and pretreated with ISO-1 (50 μm) or DMSO for 1 hr, prior to addition of Pam3CSK4 (a & b) or MALP-2 (c & d) at the concentrations indicated for a further 24 hr, with ISO-1 remaining present throughout the time course. Cell-free supernatants were collected and TNF-α (a & c) or CXCL8 (b & d) production determined by ELISA. Data are mean ± SEM from four to five separate experiments from different donors. Significant inhibition of cytokine release is indicated by *P < 0·05; **P < 0·01; analysed by two-way anova with Bonferroni's post-test.