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Variations in the numbers of DN (CD4− CD8−) subsets defined by CD44/CD25 expression. Both DN3 (CD44− CD25+) and DN4 (CD44− CD25−) cell numbers significantly decreased in EphB2 (B2−/−)-, EphB3 (B3−/−)- and EphB2/B3 (B2/B3−/−)-deficient mice.