Skip to main content
. 2008 Sep;173(3):892–900. doi: 10.2353/ajpath.2008.080001

Figure 7.

Figure 7

Increased BBB permeability in VWFKO mice is independent of encephalitogenic T cells. BBB permeability in CFA+PTX immunized WT and VWFKO mice was determined as described in Materials and Methods and was compared with BBB permeability in MOG35–55+CFA+PTX immunized WT (A), VWFKO mice (B) (n = 8 to 10 for each strain at each time point). Two-way analysis of variance revealed no difference in BBB permeability by treatment (F = 0.58; P = 0.45) or over time (F = 2.60; P = 0.08) in WT animals. Similarly, regression analysis also indicated that there is no significant difference in BBB permeability by treatment (F = 0.56; P = 0.46) or over time (F = 0.12; P = 0.73) in WT mice. In VWFKO mice, while two-way analysis of variance suggested a significant difference in BBB permeability over time (F = 6.97; P = 0.003) but not by treatment (F = 1.27; P = 0.27), regression analysis revealed no significant difference by treatment (F = 0.05; P = 0.83) or over time (F = 1.52; P = 0.23). Since there was no difference in BBB permeability between MOG35–55+CFA+PTX and CFA+PTX immunized animals, the data were pooled and re-analyzed (C). By two-way analysis of variance, changes in BBB permeability differ significantly between WT and VWFKO mice (F = 13.15; P = 0.0005) and over time (F = 5.98; P = 0.004). Regression analysis also revealed that the BBB permeability is significantly higher in VWFKO mice (F = 10.08; P = 0.001) but do not differ over time (F = 3.45; P = 0.07).