Figure 2.

The nup116-C lethal strain exhibits NPC-associated herniations of the nuclear envelope. SWY27 cells harboring the galactose-inducible Nup116-C plasmid (pSW171) were grown in SM-trp 2% raffinose at 23°C and then shifted to SM-trp 2% galactose (A and B) or SM-trp 2% glucose (C) for 15 h. Cells were subsequently processed for thin section electron microscopy. The arrowheads in A and B point to representative nuclear membrane herniations that are attached to NPC-like structures at the nuclear base. The arrows in C indicate wild-type NPCs. Bar, 250 nm.