Figure 3.

The S. cerevisiae SNL1 gene. (A) A fragment sufficient in high copy for rescuing the nup116-C phenotype was isolated from a yeast genomic library plasmid (pSW807). The library insert contained two complete and two partial ORFs from a region of chromosome IX. The ORF in pSW573 was designated SNL1 (in the yeast genome database as accession number YIL016W). + indicates the plasmid suppressed the nup116-C lethal phenotype, whereas − represents no suppression. The 2μ represents high-copy vector; CEN, low-copy vector. (B) The 159-residue amino acid sequence of the protein encoded by SNL1. A putative transmembrane segment is underlined. (C) Kyte-Doolittle (1982) hydropathy analysis of Snl1p revealed a span of sequence from amino acids 16 to 35 with significant hydropathic character.