Figure 5.

Snl1p is localized to the nuclear envelope/endoplasmic reticulum. snl1Δ cells alone (bottom panels) or harboring a high-copy plasmid expressing a protein A–Snl1p fusion (top panels) and wild-type W303α cells (middle panels) were grown to early log phase in YPD at 30°C before they were fixed in methanol/formaldehyde as described (see MATERIALS AND METHODS). Fixed cells were incubated with rabbit anti-mouse IgG (αIgG) or affinity- purified rabbit polyclonal antibodies raised against Snl1p (αSnl1p) as designated. Binding was detected with an FITC-conjugated goat anti-rabbit IgG. Perinuclear and peripheral staining was observed in wild-type cells, but not in cells lacking Snl1p. The DAPI staining delineating the location of the nucleus is shown on the right. Bar, 2.5 μm.