Figure 6.

Double immunofluorescence comparison of Snl1p localization to Pom152p and peripheral nucleoporins. Diploid W303 cells were processed for indirect immunofluorescence microscopy as described in MATERIALS AND METHODS. Fixed cells were incubated with affinity-purified rabbit polyclonal anti-Snl1p antibodies (αSnl1p) and with mouse monoclonal anti-Pom152p antibodies (αPom152p, mAb118C3) or mouse monoclonal anti-nucleoporin antibodies (mAb414). The antibodies were detected with Texas red goat anti-rabbit IgG and FITC-labeled goat anti-mouse IgG. Identical fields of cells are shown in A and B. Bar, 3 μm.