Table 1.
Plasmids used in this study
| Plasmid | Construction | Reference |
|---|---|---|
| pBJ382 Backbone | ||
| pSW169 | Fragment encoding amino acids 215–591 of NUP145 locus in BamHI–SacI | This study |
| pSW170 | Fragment encoding amino acids 590–959 of NUP100 locus in BamHI–SacI | This study |
| pSW171 | Fragment encoding amino acids 726–1113 of NUP116 locus in BamHI–SacI | This study |
| pSW556 | Fragment encoding amino acids 726–919 of NUP116 locus in BamHI–SacI | This study |
| pSW557 | Fragment encoding amino acids 914–1113 of NUP116 locus in BamHI–SacI | This study |
| pSW558 | Fragment encoding amino acids 914–1113 of NUP116 locus with 998–1005 replaced by G in BamHI/SacI | This study |
| pCITE Backbone | ||
| pSW691 | Full-length GLE2 with HA fused after C-terminal residue in EcoRI site | This study |
| pSW768 | Full-length NUP116 locus in BamHI/SacI | This study |
| pSW769 | Fragment encoding amino acids 181–1113 of NUP116 locus in BamHI/SacI | This study |
| pSW799 | Fragment encoding amino acids 1–919 of NUP116 locus in BamHI/SacI | This study |
| pSW801 | Fragment encoding amino acids 1–591 of NUP145 locus in BamHI/SacI | This study |
| pSW851 | Fragment encoding amino acids 5–838 of NIC96 locus in BamHI site | This study |
| pSW871 | Fragment encoding amino acids 1–180 of NUP116 locus in BamHI site | This study |
| pRS305 Backbone | ||
| pSW848 | Full-length NIC96 locus | This study |
| pRS315 Backbone | ||
| pSW75 | Full-length NUP116 locus in XbaI/SalI site | Wente et al. (1992) |
| pSW78 | Full-length NUP100 locus in XbaI/SalI site | Wente et al. (1992) |
| pSW278 | Full-length NIC96 locus in BamHI site | This study |
| pSW575 | Full-length SNL1 with NsiI site inserted before stop codon | This study |
| pSW576 | Full-length SNL1 with Protein A inserted at NsiI site of pSW575 | This study |
| pSW939 | Full-length SNL1 with SUC2 inserted at NsiI site of pSW575 | This study |
| pRS316 Backbone | ||
| pSW131 | Full-length NUP116 locus in XbaI/SalI site | Wente and Blobel (1994) |
| pSW201 | Full-length NUP100 locus and ADE3 locus | Murphy et al. (1996) |
| pSW574 | Full-length SNL1 locus in BamHI site | This study |
| pRS425 Backbone | ||
| pSW277 | Full-length NIC96 locus in BamHI site | This study |
| pSW336 | Full-length KAP95 locus in BamHI site | This study |
| pSW544 | Full-length GLE2 locus in SalI/SacI site | This study |
| pSW578 | Full-length SNL1 with protein A insert from pSW576 | |
| pSW586 | Partial p105 locus from library isolate (pSW807) in BamHI site | This study |
| pRS426 Backbone | ||
| pSW573 | Full-length SNL1 locus in BamHI site | This study |
| pLGSD5 Backbone | ||
| pSW418 | Full-length GLE2 locus in BamHI site | This study |
| pSW534 | Full-length SNL1 locus in BamHI site | This study |
| YEp13 Backbone | ||
| pSW847 | Library plasmid containing entire NIC96 locus in BamHI site | This study |
| YEp24 Backbone | ||
| pSW807 | Library plasmid containing entire SNL1 locus in BamHI site | This study |
| pSW853 | Library plasmid containing entire GLE1 locus in BamHI site | This study |
| pSW854 | Library plasmid pSW807 lacking 1885-bp BglII–BglII fragment | This study |
| pGEX-3X Backbone | ||
| pSW552 | Fragment encoding amino acids 36–159 of SNL1 locus in BamHI site | This study |
| pMAL-cRI Backbone | ||
| pSW640 | Fragment encoding amino acids 36–159 of SNL1 locus in BamHI site | This study |
Vector backbone references for pBJ382 for GAL-inducible GST fusion proteins (generous gift from C. Hug, Washington University, St. Louis, MO); pCITE (Novagen); pRS305, pRS315, pRS316, pRS425, and pRS426 (Sikorski and Hieter, 1989); pLGSD5 (Guarente, 1982); YEp13 (Nasmyth and Tatchell, 1980); YEp24 (Carlson and Botstein, 1982); pGEX-3X (Pharmacia); pMAL-cRI (Maina et al., 1988).